PediatricBiome Profile, Stool
Orderable by Ontario ND
Test
Clinical Utility
The US BioTek PediatricBiome Profile is a stool-based test for children and adolescents ages 3–17 years that uses PCR and shotgun metagenomic sequencing to assess gastrointestinal pathogens, parasites, fungi, digestive and inflammatory markers, microbial diversity, bacterial composition, short-chain fatty acids, and microbial functional pathways. The test may provide adjunctive information in the evaluation of gastrointestinal symptoms, digestive function, or possible infectious exposures when interpreted alongside clinical history, growth, diet, medication exposure, and standard pediatric assessment. The profile is designed to characterize gut microbial patterns and evaluate selected gastrointestinal biomarkers using age-specific reference ranges.
Method
Automated Chemiluminescent Immunometric Assay
Method Description
Automated Chemistry/Immunochemistry, Chemiluminescence Immunoassay (CLIA), Enzyme-Linked Immunosorbent Assay (ELISA),
Microscopy, Fluorescence Enzyme Immunoassay (FEIA), pH Electrode, Gas Chromatography-MS (GC/MS), Metagenomic Next Generation
Sequencing (mNGS), Quantitative PCR (qPCR), Polymerase Chain Reaction (PCR)
Result Included
- Stool Color
- Stool Form
- Mucous
- Occult Blood
- Short Chain Fatty Acid
- Beneficial
- Butyrate
- Acetate
- Propionate
- Valerate
- Calprotectin
- Pancreatic Elastase
- Secretory IgA
- Zonulin
- b-Glucuronidase
- Steatocrit
- a-Transglutaminase IgA
- pH Level
- Cryptosporidium Species
- Entamoeba Histolytica
- Giardia Intestinalis
- Blastocystis hominis
- Dientamoeba Fragilis
- Enterocytozoon species
- Cyclospora cayetanensis
- Blastocystis hominis Subtype 1
- Blastocystis hominis Subtype 2
- Blastocystis hominis Subtype 3
- Blastocystis hominis Subtype 4
- Blastocystis hominis Subtype 5
- Blastocystis hominis Subtype 6
- Blastocystis hominis Subtype 7
- Blastocystis hominis Subtype 8
- Blastocystis hominis Subtype 9
- Ascaris lumbricoides, Roundworm
- Trichuris trichiura
- Whipworm
- Enterocytozoon spp
- Strongyloides spp
- Roundworm
- Necator americanus
- Hookworm
- Enterobius vermicularis
- Pinworm
- Hymenolepis spp
- Tapeworm
- Taenia species
- Tapeworm
- Aeromonas hydrophila
- Campylobacter sp.
- C. difficile, Toxin A
- C. difficile, Toxin B
- Enteroaggregative E. coli
- Enteropathogenic E. coli
- E. coli O157
- Hypervirulent Clostridium difficile
- Enterotoxigenic E. coli LT/ST
- Salmonella sp.
- Shiga toxigenic E.coli
- Shigella spp.
- Vibrio species
- Yersinia species
- Helicobacter pylori
- Desulfovibrio piger
- Gene: A2142C
- Gene: A2142G
- Gene: A2143G
- H.pylori
- Virulence Factor, babA
- H.pylori Virulence Factor, dupA
- H.pylori Virulence Factor, oipA
- H.pylori Virulence Factor, virB
- H.pylori Virulence Factor, cagA
- H.pylori Virulence Factor, iceA
- H.pylori Virulence Factor, vacA
- H.pylori Virulence Factor, virD
- H.Pylori antigen
- Adenovirus 40/41
- Norovirus GI/II
- Rotavirus A
- Sapovirus (I,II,IV,V)
- Astrovirus (hAstro)
- Candida albicans
- Candida dubliniensis
- Candida famata
- Candida glabrata
- Candida guilliermondii
- Candida intermedia
- Candida kefyr
- Candida krusei
- Candida lambica
- Candida lipolytica
- Candida lusitaniae
- Candida parapsilosis
- Candida tropicalis
- Geotrichum sp.
- Saccharomyces cerevisiae.
- Rhodotorula sp.
- Microbial Phyla Distribution
- Ratios Firmicutes/Bacteroidetes
- Fusobacterium nucleatum/Faecalibacterium prausnitzii
- Gram-positive/Gram-negative
- Prevotella/Bacteroids
- Proteobacteria/Actinobacteria
- Personalized suggestions
- Shannons Index
- Beneficial Flora x 31 Targets
- Pathogens and Opportunistic Bacteria x 50 Targets
- Antibiotic Resistance Genes x 22 Targets
- Microbiome Function
- Carbohydrate Metabolism
- Lipid Metabolism
- Protein and Other Energy Metabolism
- Iron and Other Ion Metabolism
- LPS
- Sulfate Production
- Aging Factor Oxidative Stress
- SCFA Production
- Histamine
- Ammonia(urease)
- BCAAs Production
Test Location
US BioTek
Specimen
Specimen Type
Stool
Containers
Collection Containers
3 stool containers
Collection & Handling
Collection Instructions
Stool should be collected without contamination from urine or toilet water, and samples are obtained from multiple areas of the stool specimen.
The kit includes the following collection tubes/vials:
- Green (filled and shaken after collection)
- Red (filled but not shaken)
- Orange (collected using a swab coated lightly with stool before sealing)
Samples are placed into the provided biohazard bag with the requisition form and shipped at ambient temperature the same day if possible; if delayed, specimens should be refrigerated overnight and shipped the following day. Samples should not be frozen.
The instructions also advise avoiding antibiotics, antifungals, probiotics, laxatives, NSAIDs, and certain supplements for specified periods before collection unless otherwise directed by the ordering practitioner.
Additional Information
ICL Integrative Laboratory Services Clients: A specimen collection kit is available for registered ILS Clients with active accounts – you may login and order kits online here. Or you may register for an ILS Account here.
Stability
| Ambient | Refrigerated |
|---|---|
| 13 days | 15 days |
DO NOT freeze
Rejection Criteria
| Criteria | Specification |
|---|---|
| Specimen | Frozen |
Sample Report
Turnaround Time
18 days
Sample Report
Test Version
Last Updated
2026-05-13